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[EDIT] Previous reply was written while I was typing this] I’d also be interested in the answer. I use a Nikon D750 DSLR, which reportedly has a Sony IMX-128-(L)-AQP CMOS sensor. I usually shoot about 30 dark frames at the same exposure as my lights. I also shoot about 30 each Flats and Dark Flats. I have never bothered with Bias frames. I stack with AstroPixelProcessor which creates Masters from the calibration frames. I was just reading a long thread on Astrobin on the whole subject – in which various people announce, with what sounds like great authority … completely contradictory things.
Surely there must be a thorough scientific explanation somewhere that everyone can agree on?
By chance I photographed M101 on 2023 April 20, and had a go at it again last night, May 20. My processing isn’t particularly consistent but the earlier absence is clear.
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